Central Instrumentation Facility

Name of the Equipment

Light Emitting Diode Fluorimeter (Uranium Analyser)

Model Name M/s UA-01
Make Quantalase
Specification Detector -   Photomultiplier tube
Excitation source -   LEDs
Wavelength of emission -   As required by the user. Wavelength can be selected between 200nm to 500 nm.
Bandwidth of emission -   Typically 10-20 nm.
Pulse duration -   Between 2 to 50 microsecs. To be set by operater.
Pulse repetition rate -   1 KHzs.

Description: Fluorimeter

Major Applications  
Details of Sample Analysis Fluorimeters which use banks of pulsed LEDs to excite fluorescence in sample under study. The wavelength, pulse duration and peak power of the LED output can be set to match the excitation requirements of the sample.  The fluorescence is detected by a pulsed photomultiplier. Suitable filters after the LEDs and before the photomultiplier tube prevent LED light from reaching the photomultiplier tube directly. The filters can be broad band coloured glass filters or multilayer narrow band filters. The instrument is controlled by a microcontroller which pulses the LEDs and photomultiplier tube.

Name of the Equipment

Phase Contrast Microscope

Model Name Eclips 201, 2012
Make M/s Nikkon
Specification Episcopic illumination models: ECLIPSE LV150N (manual control types)

Phase Contrast Microscope

Major Applications Phase contrast, by "converting" phase specimens such as living material into amplitude specimens, allowed scientists to see details in unstained and/or living objects with a clarity and resolution never before achieved.
Details of Sample Analysis Unstained specimens that do not absorb light are called phase objects because they slightly alter the phase of the light diffracted by the specimen, usually by retarding such light approximately 1/4 wavelength as compared to the un deviated direct light passing through or around the specimen unaffected. Unfortunately, our eyes as well as camera film, are unable to detect these phase differences. To reiterate, the human eye is sensitive only to the colors of the visible spectrum (variations in light frequency) or to differing levels of light intensity (variations in wave amplitude). In phase specimens, the direct zeroth order light passes through or around the specimen undeviated. However, the light diffracted by the specimen is not reduced in amplitude as it is in a light-absorbing object, but is slowed by the specimen because of the specimen's refractive index or thickness (or both). This diffracted light, lagging behind by approximately 1/4 wavelength, arrives at the image plane out of step (also termed out of phase) with the undeviated light but, in interference, essentially undiminished in intensity. The result is that the image at the eyepiece level is so lacking in contrast as to make the details almost invisible.

Name of the Equipment

Laminar Hood

Model Name Vertical Laminar Flow system
Make M/s Alpha Linear
Specification Pre filter (90% down to 10 micron)
HEPA filter (99.97% down to 0.3 micron)
Front Side door
Polycarbonate/Glass window
Manual Operation/ Counterweight Mechanism
UV light
5A Power Socket
Differential Pressure Gauge


Laminar Hood

Major Applications 1. Laminar flow cabinets are used in laboratories for contamination sensitive processes like plant tissue culture.
2. Other laboratories processes like media plate preparation and culture of organisms can be performed inside the cabinet.
3. Operations of particle sensitive electronic devices are performed inside the cabinet.
Details of Sample Analysis Microbial Load

Name of the Equipment

Bacteriological Incubator

Model Name ACM 22062-1
Make ACMAS, Pvt.Ltd.
Specification An incubator is an insulated and enclosed device used in biological laboratories. It creates an optimum environment which is required for the growth of microorganisms by providing optimum temperature, humidity, and other environmental conditions such as the CO2 and oxygen content inside’s atmosphere. 

Bacteriological Incubator

Major Applications it is used to grow and maintain microbiological cultures or cell cultures. Both bacterial and eukaryotic cell organisms are cultivated by using an incubator.
Details of Sample Analysis Bacterial Culture

Name of the Equipment

UV- Vis Spectrophotometer

Model Name Genesys-50
Make M/s. Thermo Fisher
Specification
Accuracy (Photometric) 3% (at 0.97 A)
Certifications/Compliance All NanoDrop instruments are approved to CE and UL/CSA.
Compatibility DYMO LabelWriter 450 printer, Bluetooth keyboard, mouse and barcode reader
Connections 1.Three USB-A ports, Ethernet, Bluetooth™ and Wi-Fi
2.(Only available on instruments with Wi-Fi/Bluetooth support)
Detector Type 2048-element CMOS linear image sensor
Display 7-inch, 1280 x 800 high-definition color display
Operating System Android™
Photometric Accuracy Instrument 1. 3% at 0.97 A, 302 nm
2. (Absorbance expressed at Abs/mm at 25°C)
Printer External Printer: DYMO Label Writer 450
System Requirements Windows™ 8.1 and 10, 64 bit
Wavelength Accuracy ±1 nm
Wavelength Range 190–850 nm
Applications 1. Nucleic Acid A260, A260/A280, A260/A230 and Labeled Nucleic Acids;
2. Protein A280 and A205, Protein Pierce 660, Protein Bradford, Protein BCA, Protein Lowry, Labeled Proteins, OD600, Kinetics, UV-Vis, and Custom Methods
Concentration 1. Maximum
2. dsDNA: Pedestal: 27,500 ng/µL
3. BSA (IgG): Pedestal: 820 (400) mg/mL
Description NanoDrop One Microvolume UV-Vis Spectrophotometer with Wi-Fi
Detection Range 1. dsDNA: Pedestal: 2.0 ng/µL; Cuvette: 0.2 ng/µL
2.BSA (IgG): Pedestal: 0.06 (0.03) mg/mL; Cuvette: 0.006 (0.003) mg/mL
Dimensions (L x W x H) 20 x 25.4 x 32.3 cm (8 x 10 x 12.7 in.)
Keypad Built-in touchscreen, multipoint capacitive touch
Lamp Xenon flash lamp


UV- Vis Spectrophotometer

Major Applications -Quantify and qualify DNA, RNA, and protein samples.
-Identify sample contaminants and obtain corrected concentration results.
Details of Sample Analysis Biological samples

Name of the Equipment

Thermal Cycler PCR

Model Name T 100 Thermal cycler
Make M/s. Biorad
Specification
Thermal Cycler S1000
Input power, W, maximum 700
Frequency, Hz, single phase 50–60
Display (LCD) Yes
Temperature control modes Calculated and block
PCR license Yes
PC compatibility (Windows XP or higher) Yes
Faster ramping: 96-well: 0.2 ml
Higher volume: 96–deep well: 0.2 and 0.5 ml
Accuracy: ±0.2°C
Uniformity: ±0.4°C well-to-well within 10 sec
Max ramp rate: up to 5°C/sec
Temperature range: 0–100°C


Thermal Cycler PCR

Major Applications Amplification/PCR
Cloning
Cycle sequencing
Gene expression studies
Mutagenesis
Details of Sample Analysis Biological samples

Name of the Equipment

Elisa Reader and Washer

Model Name iMark
Make M/s. Biorad
Specification Wavelength range - 400–750 nm
Photometric range- 0.0–3.5 OD
Linearity - ≤1.0% from 0.0–2.0 OD; ≤2.0% from 0.0–3.0 OD
Accuracy- ≤1.0% or 0.010 from 0.000–3.000 OD at 490 nm
Precision - 1.0% or 0.005 OD from 0.0–2.0 OD; 1.5% from 2.0–3.0 OD
Resolution- 0.001 OD
Filter wheel capacity- 8
Plate shaking (3 speeds)- Low, mid, high
Duration, sec- 0–999
Read time - 6 sec at single wavelength, 10 sec at dual wavelengths
Data output - Onboard graphical thermal printer and USB2 interface with PC or Mac data stations
Data storage - Calender/clock functions; 64 assay protocols
Multilanguage support - 4 languages, LCD indication supported; printout report supported
Dimensions (W x D x H), cm (in)- 34.6 x 37.7 x 16.4 (13.6 x 14.8 x 6.5)
Weight, kg (lb)- 5.5 (12)


Elisa Reader and Washer

Major Applications Immunological Studies
Details of Sample Analysis Biological samples

Name of the Equipment

Refrigerated Centrifuge

Model Name Sorvall legend micro.17R
Make M/s. Biorad
Specification ID-Centrifuge 12 S II is a silent centrifuge for up to 12 ID-Cards with automatic balance control. The centrifugation time is 10 minutes.
Speed: 1030 rpm (85 g)
Dimensions (w/h/d): 30 cm / 18 cm / 36 cm
Weight: 6.2 kg
Power requirements: 100-240 V / 50-60 Hz


Refrigerated Centrifuge

Major Applications • Separation of mixtures with close densities.
• Separate immiscible liquids.
• Sediment suspended solids.
• Separation of blood.
• Separation insoluble particles (e.g. insoluble proteins in a protein solution)
• Isotope Separation.
Details of Sample Analysis Biological samples

Name of the Equipment

Horizontal and Vertical Electrophoresis unit

Model Name Mini-Protrean Tetra cell and Mini-Sub cell GT Respectively
Make M/s. Biorad
Specification Number of gels
1–4
Precast gels
Ready Gel precast gels
Handcast gels
Cast using Mini-PROTEAN spacer plates
Gel size (W x L)
Precast: 8.6 x 6.8 cm Handcast: 8.3 x 7.3 cm
Glass plate size (W x L)
Short plate
10.1 x 7.3 cm
Spacer plate
10.1 x 8.2 cm
Total buffer volume for 2 gels
700 ml
Total buffer volume for 4 gels
1,000 ml
Typical run times for SDS-PAGE
35–45 min (at 200 V constant)
Recommended power supply
PowerPac Basic or PowerPac HC (High Current)
Dimensions (W x L x H)
12 x 16 x 18 cm


Horizontal and Vertical Electrophoresis unit

Major Applications horizontal gel electrophoresis is an ideal choice for DNA and RNA separation, while vertical systems are ideal for proteins
Details of Sample Analysis DNA, RNA and Proteins

Admission 2021-22